4ZYJ
Streptomyces peucetius nitrososynthase dnmZ in TDP-bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.483, 134.667, 144.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.426 - 2.736 |
R-factor | 0.1766 |
Rwork | 0.173 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mxl |
RMSD bond length | 0.013 |
RMSD bond angle | 1.762 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.426 | 39.426 | 2.880 |
High resolution limit [Å] | 2.736 | 8.650 | 2.736 |
Rmerge | 0.041 | 0.012 | 0.350 |
Rmeas | 0.045 | ||
Rpim | 0.018 | 0.005 | 0.170 |
Total number of observations | 325167 | 10462 | 33667 |
Number of reflections | 51414 | ||
<I/σ(I)> | 32.9 | 104.1 | 4.1 |
Completeness [%] | 98.2 | 98.4 | 88.9 |
Redundancy | 6.3 | 5.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 298 | 0.1 M glycine, 0.12 M ammonium sulfate, 12% PEG2000, pH 9.4 |