4ZYA

The N-terminal extension domain of human asparaginyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-23
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	32.626, 32.626, 215.921
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.016 - 1.650
R-factor	0.2105
Rwork	0.206
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge		0.497
Number of reflections	14574
<I/σ(I)>	17.9	5.1
Completeness [%]	97.1	99.1
Redundancy	11.7	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	zinc sulfate, MES, PEG 550 MME