4ZY7
Crystal structure of a Mycobacterial protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 42.610, 70.490, 100.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.220 - 1.700 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nss |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.065 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.220 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.337 |
| Number of reflections | 33734 | |
| <I/σ(I)> | 20.9 | 4.8 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | 16% PEG 6000, 0.1M Tris-HCl pH 8.3, 0.2M magnesium chloride |
