4ZXW
Crystal structure of SgcC5 protein from Streptomyces globisporus (complex with (R)-(-)-1-(2-naphthyl)-1,2-ethanediol and sucrose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.397, 105.304, 108.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.278 - 2.187 |
| R-factor | 0.1615 |
| Rwork | 0.161 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4znm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.386 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1938) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.109 | 0.762 |
| Number of reflections | 58829 | |
| <I/σ(I)> | 14.8 | 2.36 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.2 M lithium sulfate, 0.1 M CAPS:NaOH, pH 10.5, 2 M ammonium sulfate, 4.06 mM (S)-beta-tyrosine-N-acetylethylenediamine, 4.06 mM (R)-(-)-1-(2-naphthyl)-1,2-ethanediol, cryo 28% sucrose |
