4ZXS
HSV-1 nuclear egress complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.60000 |
| Spacegroup name | P 6 |
| Unit cell lengths | 110.529, 110.529, 155.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.860 - 2.772 |
| R-factor | 0.2195 |
| Rwork | 0.217 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z3u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.860 | 2.870 |
| High resolution limit [Å] | 2.772 | 2.772 |
| Rmerge | 0.081 | 0.559 |
| Number of reflections | 27365 | |
| <I/σ(I)> | 34.37 | 4.57 |
| Completeness [%] | 99.8 | 97.8 |
| Redundancy | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na citrate pH 5.6, 5 mM NiCl2, 10% PEG8000 |
