4ZXO
The structure of a GH26 beta-mannanase from Bacteroides ovatus, BoMan26A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.856, 79.434, 87.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.272 - 1.500 |
| R-factor | 0.1381 |
| Rwork | 0.136 |
| R-free | 0.17770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vx4 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.611 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.272 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | |
| Number of reflections | 52731 | |
| <I/σ(I)> | 18.24 | 2.53 |
| Completeness [%] | 99.8 | 99.27 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50 mM MES buffer, 0.1 M potassium thiocyanate, 30% (w/v) PEG monoethyl ether 2000 |
