4ZXN
Crystal structure of rat coronavirus strain New-Jersey Hemagglutinin-Esterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.710, 184.370, 76.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.490 - 2.200 |
| R-factor | 0.23412 |
| Rwork | 0.232 |
| R-free | 0.27826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BCoV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.925 |
| Data reduction software | MOSFLM (7.09) |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.490 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.109 | 0.683 |
| Number of reflections | 22281 | |
| <I/σ(I)> | 8.5 | 2.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Bis Tris propane pH 7.5, 0.2M Sodium fluoride, 20% w/v PEG 3350 |
