4ZXM
Crystal structure of PGRP domain from Branchiostoma belcheri tsingtauense peptidoglycan recognition protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97886 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.596, 79.596, 82.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.19767 |
| Rwork | 0.197 |
| R-free | 0.21948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oht |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 7646 | |
| <I/σ(I)> | 8.4 | 3.1 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 1.8 M Sodium Chloride, 0.1 M citric acid |
