4ZVC
Crystal structure of MID domain of the E. coli DosC - form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.220, 43.570, 52.440 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 48.325 - 1.500 |
| R-factor | 0.1495 |
| Rwork | 0.147 |
| R-free | 0.18860 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.059 | 0.380 |
| Number of reflections | 35038 | |
| <I/σ(I)> | 18.57 | 5.37 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Bis-Tris pH 5.5, 0.2M magnesium chloride, 17% (w/v) PEG 3350, 0.1M Gd-HPDO3A [gadolinium complex of 10-(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid] |
