4ZUI
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-04-15 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.096, 60.419, 38.079 |
| Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
| Resolution | 32.150 - 1.700 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.827 |
| Data reduction software | XPREP |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.025 | 0.172 |
| Total number of observations | 209747 | |
| Number of reflections | 15518 | |
| <I/σ(I)> | 29.44 | 5.34 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.21 | 7.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
