4ZTY
Neurospora crassa cobalamin-independent methionine synthase complexed with Cd2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.54980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.045, 95.198, 115.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.020 - 1.880 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.20180 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.7) |
| Phasing software | REFMAC (5.5.0109) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.020 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.122 | 1.013 |
| Number of reflections | 494614 | |
| <I/σ(I)> | 11.7 | 1.8 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% w/v PEG3350, 200 mM potassium nitrate, 1 mM TCEP, 20% v/v glycerol |
