4ZTO
Fab/epitope complex structure of rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.283, 84.831, 167.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.143 - 2.300 |
R-factor | 0.1698 |
Rwork | 0.167 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jo1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.073 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 48392 | |
<I/σ(I)> | 18 | 3.7 |
Completeness [%] | 99.1 | 97.5 |
Redundancy | 6.5 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 24% polyethylene glycol 6000, 0.1 M citric acid, and 0.02% NaN3 |