4ZTC
PglE Aminotransferase in complex with External Aldimine, Mutant K184A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-25 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.161, 66.161, 169.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 2.000 |
| R-factor | 0.19184 |
| Rwork | 0.188 |
| R-free | 0.25445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1O61 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.715 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.237 |
| Number of reflections | 26017 | |
| <I/σ(I)> | 15.1 | 2.8 |
| Completeness [%] | 99.9 | 97.4 |
| Redundancy | 13.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 15-18% PEG3350, 1 mM PLP, 10 mM UDP-2-acetamido-4-amino-2,4,6-trideoxyglucose, 100 mM MOPS, 200 mM tetraethylammoniumchloride |
