4ZT1
Crystal structure of human E-Cadherin (residues 3-213) in x-dimer conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.446, 76.417, 73.020 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 42.650 - 1.920 |
| R-factor | 0.1941 |
| Rwork | 0.193 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o72 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.507 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.045 | 0.253 |
| Number of reflections | 48085 | |
| <I/σ(I)> | 8.4 | 2.2 |
| Completeness [%] | 86.4 | 58.5 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | TRIS 0.1 M, ammonium sulfate 1.3 M, calcium chloride 50 mM |
