4ZSZ

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-3.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-07-16
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.542
Spacegroup name	P 31 2 1
Unit cell lengths	112.930, 112.930, 150.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.492 - 4.251
R-factor	0.2658
Rwork	0.265
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2arh 3kaw
RMSD bond length	0.005
RMSD bond angle	0.859
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.927		4.360
High resolution limit [Å]	4.250	19.010	4.250
Rmerge	0.115	0.023	0.808
Rmeas	0.128	0.028	0.905
Total number of observations	43354
Number of reflections	8061	82	556
<I/σ(I)>	12.66	40.89	2.03
Completeness [%]	98.9	73.9	94.6
Redundancy	5.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1M Bis-Tris (pH=5.5) and 1.5M ammonium sulfate