4ZSK
Crystal structure of the effector-binding domain of DasR (DasR-EBD) in complex with N-acetylglucosamine-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 54.211, 54.211, 222.512 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.298 - 1.850 |
R-factor | 0.1946 |
Rwork | 0.192 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Effector-binding domain of entry 2wv0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.113 | 1.122 |
Number of reflections | 33544 | |
<I/σ(I)> | 11.7 | 1.8 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 7.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M potassium thiocyanate and 30 % (w/v) PEG monomethyl ether 2,000 |