4ZS9
Raffinose and panose binding protein from Bifidobacterium animalis subsp. lactis Bl-04, bound with raffinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.540, 90.820, 146.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.000 - 1.370 |
| R-factor | 0.1341 |
| Rwork | 0.132 |
| R-free | 0.16660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zza |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 1.410 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.080 | 0.775 |
| Number of reflections | 151108 | |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 96.9 | 94.1 |
| Redundancy | 6.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 278 | 0.1 M Tris pH 8.5, 25% PEG 4000, 0.8 M magnesium chloride |
