4ZQV

CdiI Immunity protein from Yersinia kristensenii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	54.448, 54.448, 54.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.584 - 1.803
R-factor	0.1853
Rwork	0.181
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6u
RMSD bond length	0.007
RMSD bond angle	0.998
Data reduction software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	16618
<I/σ(I)>	9.48
Completeness [%]	99.7
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.2 M Ammonium Sulfate, 20% PEG 3350