4ZQU
CdiA-CT/CdiI toxin and immunity complex from Yersinia pseudotuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.510, 68.310, 71.419 |
Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
Resolution | 46.490 - 2.090 |
R-factor | 0.2112 |
Rwork | 0.205 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g6u |
RMSD bond length | 0.003 |
RMSD bond angle | 0.694 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.490 |
High resolution limit [Å] | 2.090 |
Number of reflections | 18152 |
<I/σ(I)> | 10 |
Completeness [%] | 99.4 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM HEPES, pH 7, 20% PEG 3350, 1% tryptone |