4ZQA
Crystal Structure of the Sds3 Dimerization Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856, 0.97872 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.460, 49.390, 106.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.650 |
| R-factor | 0.16257 |
| Rwork | 0.160 |
| R-free | 0.20896 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.307 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.045 | 0.246 |
| Number of reflections | 13534 | |
| <I/σ(I)> | 11.3 | 2.8 |
| Completeness [%] | 94.2 | 96.5 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 287 | 15% ethanol (v/v), 200 mM MgCl2, and 100 mM imidazole |
