4ZQ8
Crystal structure of a terpene synthase from Streptomyces lydicus, target EFI-540129
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.737, 153.781, 155.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.843 - 2.000 |
| R-factor | 0.1963 |
| Rwork | 0.193 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4la6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.103 | 0.040 | 0.487 |
| Rmeas | 0.123 | 0.048 | 0.580 |
| Rpim | 0.065 | 0.025 | 0.307 |
| Total number of observations | 151695 | ||
| Number of reflections | 50304 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 93.8 | 91.5 | 93.4 |
| Redundancy | 3 | 3.1 | 3.1 |
| CC(1/2) | 0.996 | 0.853 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | Protein (43 mg/ml, 10 mM Hepes pH 7.5); Reservoir (0.1 M Bicine pH 9.0, 20% PEG 6000); cryo (20% glycerol) |
