4ZPC
Coxsackievirus B3 Polymerase - A341G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-31 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.289, 74.289, 288.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.763 - 1.590 |
R-factor | 0.2132 |
Rwork | 0.213 |
R-free | 0.22960 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.851 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.763 | 5.960 | 2.110 |
High resolution limit [Å] | 1.590 | 4.220 | 1.990 |
Rmerge | 0.274 | 0.252 | 0.574 |
Rmeas | 0.296 | 0.270 | 0.662 |
Total number of observations | 656402 | ||
Number of reflections | 203130 | 7687 | 15450 |
<I/σ(I)> | 4.66 | 8.12 | 1.08 |
Completeness [%] | 96.7 | 100 | 84.2 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.085 M HEPES pH 7.5, 3.6 M NaCl , 15% Glycerol |