4ZP8

Coxsackievirus B3 Polymerase - F364L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.221, 74.221, 289.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.482 - 1.894
R-factor	0.2007
Rwork	0.200
R-free	0.23270
RMSD bond length	0.007
RMSD bond angle	0.816
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.482		2.010
High resolution limit [Å]	1.894	5.660	1.890
Rmerge	0.145	0.056	1.224
Rmeas	0.156	0.060	1.348
Total number of observations	851797
Number of reflections	122235	4644	19184
<I/σ(I)>	9.39	26.87	1.15
Completeness [%]	99.4	99.7	96.2
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.085 M Tris, 1.2 M Ammonium Sulfate , 25% Glycerol