4ZOU
Crystal structure of the BTB domain of SLX4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 96.391, 96.391, 71.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.739 - 2.150 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24440 |
| Structure solution method | SAD |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.089 | 0.037 | 0.808 |
| Total number of observations | 187864 | ||
| Number of reflections | 11151 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.5 | 99.3 | 96.1 |
| Redundancy | 16.8 | 15.9 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 277 | 4-6% PEG 6000, 0.1 M HEPES, pH 7.6, 5% glycerol |
