4ZOU

Crystal structure of the BTB domain of SLX4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	96.391, 96.391, 71.825
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.739 - 2.150
R-factor	0.203
Rwork	0.201
R-free	0.24440
Structure solution method	SAD
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.089	0.037	0.808
Total number of observations	187864
Number of reflections	11151
<I/σ(I)>	7.1
Completeness [%]	99.5	99.3	96.1
Redundancy	16.8	15.9	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	277	4-6% PEG 6000, 0.1 M HEPES, pH 7.6, 5% glycerol