4ZOL

Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	105.127, 154.824, 186.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.630 - 2.500
R-factor	0.177
Rwork	0.174
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	105685
<I/σ(I)>	18.6
Completeness [%]	99.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2