4ZOE
Crystal Structure of beta-glucosidase from Listeria innocua
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.750, 95.100, 214.996 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 28.170 - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ex1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.021 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.380 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 163230 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 98.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, NaCl, LiSO4, glycerol |
