4ZOA
Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.650, 94.850, 215.868 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 40.710 - 2.170 |
| R-factor | 0.1947 |
| Rwork | 0.191 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zoe |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.791 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.490 |
| High resolution limit [Å] | 2.170 |
| Number of reflections | 92779 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 98.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, NaCl, LiSO4, glycerol |
