4ZMX
Crystal structure of human P-cadherin (int-X-dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 181.460, 181.460, 40.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.590 - 3.100 |
| R-factor | 0.20755 |
| Rwork | 0.206 |
| R-free | 0.23538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.927 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.590 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.115 | 0.407 |
| Number of reflections | 14283 | |
| <I/σ(I)> | 3.4 | 3 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 4,000 10% 2-isopropanol HEPES 100 mM |
