4ZMN
Crystal structure of human P-cadherin (ss-dimer long)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.980, 108.980, 93.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.670 - 2.600 |
| R-factor | 0.19403 |
| Rwork | 0.192 |
| R-free | 0.24288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o72 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.569 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.670 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.111 | 0.527 |
| Number of reflections | 10512 | |
| <I/σ(I)> | 13.7 | 3.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 550-MME 100 mM CaCl2 100 mM HEPES |
