4ZMK
Crystal structure of the dimerization domain of S. pombe Taz1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.078, 45.078, 78.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.641 - 1.500 |
| R-factor | 0.1625 |
| Rwork | 0.159 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.796 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.046 | 0.037 | 0.271 |
| Total number of observations | 85864 | ||
| Number of reflections | 13259 | ||
| <I/σ(I)> | 18.2 | ||
| Completeness [%] | 97.1 | 91.5 | 85.9 |
| Redundancy | 6.5 | 6.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100mM Bicine pH8.5, 2.3M Ammonium Sulfate, 10mM DTT |
