4ZLX

N-terminal DNA binding domain of the antitoxin Phd from phage P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-19
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	42.940, 42.940, 100.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.860 - 2.310
R-factor	0.1817
Rwork	0.179
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hs2
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	2.390
High resolution limit [Å]	2.310	2.310
Rmerge		0.130
Number of reflections	4518
<I/σ(I)>	57.8	17.9
Completeness [%]	97.3	86.7
Redundancy	11.4	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM Lithium Sulfate monohydrate, 20% w/v PEG3350