4ZLL
Crystal structure of transporter AcrB triple mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 146.709, 146.709, 523.924 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.902 - 3.360 |
| R-factor | 0.2484 |
| Rwork | 0.245 |
| R-free | 0.31250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d9b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.550 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.480 | 3.540 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Number of reflections | 31534 | |
| <I/σ(I)> | 4.4 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M ADA buffer at pH 7.4, 0.1 M Li2SO4, 10% PEG 3350 |
