4ZLH
Structure of the LapB cytoplasmic domain at 2 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.28292 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.310, 152.250, 65.470 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 40.075 - 2.000 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.25450 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.100 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.064 |
| Number of reflections | 97844 |
| <I/σ(I)> | 11.01 |
| Completeness [%] | 99.7 |
| Redundancy | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M (NH4)2SO4, 25% PEG 3350, and 0.1 M Bis-Tris pH 6.5 |
