4ZKV
Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) refined to 1.92A at P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.214, 79.001, 63.876 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.920 |
| R-factor | 0.21274 |
| Rwork | 0.209 |
| R-free | 0.28662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tw2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.882 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.169 | 0.532 |
| Number of reflections | 34309 | |
| <I/σ(I)> | 5.3 | 1.6 |
| Completeness [%] | 97.8 | 98.5 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 281 | 18% PEG 4000, 0.1M sodium cacodylate pH 6.8 |
