4ZKP
P22 Tail Needle Gp26 crystallized at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 3 |
| Unit cell lengths | 43.846, 43.846, 272.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.120 - 2.100 |
| R-factor | 0.158 |
| Rwork | 0.154 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.577 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_2016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.200 |
| Number of reflections | 32511 | |
| <I/σ(I)> | 16.3 | 4.55 |
| Completeness [%] | 95.2 | 70.4 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 40% PEG 1000, 0.1M MOPS pH7.0 |
