4ZKJ

Crystal structure of CRISPR-associated protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	95.205, 95.205, 210.843
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.140 - 2.250
R-factor	0.18295
Rwork	0.182
R-free	0.21129
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.760
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.078	0.657
Number of reflections	579520
<I/σ(I)>	21.25	3.97
Completeness [%]	99.6	99.5
Redundancy	10.9	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.6/0.6M Na/KH2PO4, 27% (w/v) glycerol and 130 mM HEPES pH 7.0