4ZK9

The chemokine binding protein of orf virus complexed with CCL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	93.2
Detector technology	CCD
Collection date	2011-03-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95369
Spacegroup name	P 43 21 2
Unit cell lengths	78.802, 78.802, 185.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.540 - 2.600
R-factor	0.24062
Rwork	0.238
R-free	0.28094
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p5i
RMSD bond length	0.006
RMSD bond angle	1.194
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.540	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.067	0.893
Number of reflections	18835
<I/σ(I)>	31.9	3.1
Completeness [%]	100.0	100
Redundancy	14.2	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	0.2M potassium sodium tartrate tetrahydrate, 0.1M sodium citrate tribasic dihydrate pH5.6, 2.0M ammonium citrate