4ZJP

Structure of an ABC-Transporter Solute Binding Protein (SBP_IPR025997) from Actinobacillus Succinogenes (Asuc_0197, TARGET EFI-511067) with bound beta-D-ribopyranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54056
Spacegroup name	P 1
Unit cell lengths	36.261, 37.416, 48.165
Unit cell angles	95.20, 101.03, 97.09

Refinement procedure

Resolution	20.000 - 1.630
R-factor	0.1436
Rwork	0.141
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dri
RMSD bond length	0.006
RMSD bond angle	0.953
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.660
High resolution limit [Å]	1.630	4.410	1.630
Rmerge	0.046	0.042	0.165
Rmeas	0.057	0.053	0.204
Rpim	0.034	0.032	0.118
Total number of observations	69277
Number of reflections	26483
<I/σ(I)>	30
Completeness [%]	87.1	89.3	81.5
Redundancy	2.6	2.5	2.6
CC(1/2)		0.994	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Protein (40 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM D-ribose); Reservoir (22.7 %(w/v) PEG 3350, 0.1M BisTris pH 8.0); Cryoprotection (80% reservoir,20% ethylene glycol)