4ZJN
Crystal structure of the bacteriophage G20C portal protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 155.328, 155.328, 115.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.400 - 1.980 |
| R-factor | 0.17496 |
| Rwork | 0.175 |
| R-free | 0.20500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.064 | 0.673 |
| Number of reflections | 97710 | |
| <I/σ(I)> | 17.3 | 2.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Protein in 1 M NaCl, 10 mM HEPES pH 7.5. Reservoir: 0.2 M magnesium chloride, 40%(v/v) MPD. |
