4ZJK
FIVE MINUTES IRON LOADED HUMAN H FERRITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.800, 183.800, 183.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.070 - 1.560 |
| R-factor | 0.16883 |
| Rwork | 0.168 |
| R-free | 0.19244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ajo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.070 | 1.640 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.059 | 0.441 |
| Number of reflections | 38257 | |
| <I/σ(I)> | 25 | 3.6 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 11.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 281 | 0.1 Bicine pH 9.0, 2.0 M Magnesium Chloride |
