4ZJH
Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning domains NIE-MG1.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793, 0.9687 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.490, 32.860, 58.320 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 49.340 - 1.600 |
| R-factor | 0.1831 |
| Rwork | 0.182 |
| R-free | 0.21730 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.367 |
| Number of reflections | 24951 | |
| <I/σ(I)> | 24.1 | 4.6 |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 6.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 25% [w/v] PEG3,350 200mM ammonium acetate 100mM sodium acetate |
