4ZI3
BART-like domain of BARTL1/CCDC104 aa1-133 in complex with Arl3FL bound to GppNHp in P1 21 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.550, 67.720, 98.470 |
| Unit cell angles | 90.00, 102.65, 90.00 |
Refinement procedure
| Resolution | 28.950 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zi2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.950 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | |
| Number of reflections | 44692 | |
| <I/σ(I)> | 15.28 | 3.72 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.35 | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 1 M LiCl, 0.1 M Tris pH8.5, 20 % PEG 4000 |
