4ZHW

Crystal structure of a bacterial signalling protein (N-terminal truncation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	40.650, 40.650, 155.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.007 - 1.391
R-factor	0.181
Rwork	0.180
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zhv
RMSD bond length	0.008
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.390
Number of reflections	27019
<I/σ(I)>	27.9
Completeness [%]	99.0
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	3.5M sodium formate