4ZHV

Crystal structure of a bacterial signalling protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	36.841, 56.418, 139.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.893 - 1.585
R-factor	0.1675
Rwork	0.166
R-free	0.18820
RMSD bond length	0.009
RMSD bond angle	1.201
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.580
Number of reflections	40264
<I/σ(I)>	37.8
Completeness [%]	99.7
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 2% PEG 400, 2.0 M ammonium sulfate