4ZHS

Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Trichophyton rubrum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	157.504, 157.504, 188.401
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.621 - 2.603
R-factor	0.2158
Rwork	0.214
R-free	0.25680
RMSD bond length	0.007
RMSD bond angle	1.059
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.621	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.096	0.693
Number of reflections	311564
<I/σ(I)>	7.24	1.56
Completeness [%]	97.2	97.1
Redundancy	1.87	1.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	0.2M Ammonium Sulfate, 0.1M Sodium citrate pH5.4, 15% PEG 4000