4ZHP
The crystal structure of Potato ferredoxin I with 2Fe-2S cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.74 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.660, 71.690, 79.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.700 - 2.460 |
| R-factor | 0.17866 |
| Rwork | 0.177 |
| R-free | 0.21472 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n58 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.999 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.063 | 0.596 |
| Number of reflections | 5483 | |
| <I/σ(I)> | 13.9 | 4.3 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 12.3 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.1 M Malic acid/MES/Tris, 25 % PEG 1500 |
