4ZH6

Crystal Structure of the Domain-Swapped Dimer Y60L mutant of Human Cellular Retinol Binding Protein II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 2 21 21
Unit cell lengths	36.776, 60.424, 63.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.837 - 1.550
R-factor	0.1902
Rwork	0.187
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.006
RMSD bond angle	0.959
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.588
High resolution limit [Å]	1.550	1.550
Rmerge	0.052	0.695
Number of reflections	21293
<I/σ(I)>	82.03
Completeness [%]	99.8	100
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	25% PEG 4000 (40%), 0.1M Sodium Acetate pH 4.5, Ammonium Acetate 0.1M