4ZGV
The Crystal Structure of the Ferredoxin Receptor FusA from Pectobacterium atrosepticum SCRI1043
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.274, 79.886, 137.905 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 48.950 - 3.200 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.598 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.305 | 1.730 |
| Number of reflections | 49734 | |
| <I/σ(I)> | 7 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 11-14 % PVP, 14 % PEG 2000 MME, 0.1 Tris, 0.05 M MgCl2 pH 7.5, with a FusA concentration of 15 mg.ml-1 in 50 mM Tris, 200 mM NaCl, 0.8-1 % (v/v) beta-OG, 0.4 % LDAO pH 7.9, at 289 K |
