4ZG5
Structural and functional insights into Survival endonuclease, an important virulence factor of Brucella abortus
Replaces: 4QEAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.181, 121.068, 82.431 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.19986 |
| Rwork | 0.198 |
| R-free | 0.22725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ty2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.540 | |
| Number of reflections | 75815 | |
| <I/σ(I)> | 21.9 | 2.16 |
| Completeness [%] | 97.8 | 99.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 15% PEG 4000, TRIS, 0.2M MGCL2 |
