4ZG4
Myosin Vc Pre-powerstroke
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.280, 67.320, 133.180 |
| Unit cell angles | 90.00, 108.48, 90.00 |
Refinement procedure
| Resolution | 49.536 - 2.360 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vom |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.536 | 2.500 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.058 | 0.542 |
| Number of reflections | 81862 | |
| <I/σ(I)> | 14.25 | 2.43 |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 10% PEG8k, 50 mM MES, pH 6.5, 1% DMSO |
